Hans Törmä, Teresa Karlsson, Gerd Michaëlsson, Ola Rollman, Anders Vahlquist
DOI: 310.1080/00015550050500013

Retinoic acid, vitamin D3 and triiodothyronine regulate keratinocyte proliferation and differentiation - processes that are disturbed in psoriatic skin - via binding to nuclear receptors for retinoic acid (RAR-α,-γ), vitamin D3 (VDR), thyroid hormone (TR-α,-β) plus the common heterodimer partners, the 9-cis-retinoic acid receptors (RXR-α,-β). By using a new quantitative real-time polymerase chain reaction assay, the expression of these receptors and three housekeeping genes (cyclophilin, GAPDH and β-actin) was studied in psoriatic skin. The expression of housekeeping genes was consistently 2.7-4.3 times higher in lesional than in non-lesional skin. When the β-actin expression was used to normalize the receptor mRNA values, the RARα, RXRα and TRα transcripts were found to be 58-75% lower in lesional vs. non-lesional skin and the RXRα:RARγ ratio was reduced from 3.2 to 1.5. Topical treatment for 4 days with 0.025% all- trans -retinoic acid or calcipotriol under occlusion did not normalize the altered mRNA expression of RARs, RXR and VDR in lesional skin. The results suggest that retinoid and thyroid hormone signalling is abnormal in lesional psoriatic skin, but how this relates to the pathogenesis of the disease is still unclear.